Antibody Name:	Anti-ABCB8 Antibody
Antibody SKU:	CAB2653
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 469-718 of human ABCB8 (NP_009119.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Jurkat, HepG2, HeLa, Mouse heart, Mouse skin, Mouse kidney, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 469-718 of human ABCB8 (NP_009119.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GFEV LKDF TLTL PPGK IVAL VGQS GGGK TTVA SLLE RFYD PTAG VVML DGRD LRTL DPSW LRGQ VVGF ISQE PVLF GTTI MENI RFGK LEAS DEEV YTAA REAN AHEF ITSF PEGY NTVV GERG TTLS GGQK QRLA IARA LIKQ PTVL ILDE ATSA LDAE SERV VQEA LDRA SAGR TVLV IAHR LSTV RGAH CIVV MADG RVWE AGTH EELL KKGG LYAE LIRR QALD APRT AAPP PKKP EGPR SHQH KS
Gene ID:	11194
Uniprot:	Q9NUT2
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	59kDa/68kDa/75kDa/78kDa/79kDa
Observed MW:	80kDa

Synonyms:

ABCB8, EST328128, M-ABC1, MABC1

Background:

This nuclear gene encodes a multi-pass membrane protein that is targeted to the mitochondrial inner membrane. The encoded protein is an ATP-dependent transporter that may mediate the passage of organic and inorganic molecules out of the mitochondria. Loss of function of the related gene in mouse results in a disruption of iron homeostasis between the mitochondria and cytosol. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ABCB8: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined; however, it may involve the compartmentalization and transport of heme, as well as peptides, from the mitochondria to the nucleus and cytosol. This protein may also play a role in the transport of phospholipids into mitochondrial membranes. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Hydrolase; Transporter, ABC family; Membrane protein, integral; Mitochondrial; Transporter; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 7q36 Cellular Component: mitochondrial envelope; mitochondrion; membrane; mitochondrial inner membrane; integral to membrane; nucleolus; ATP-binding cassette (ABC) transporter complex; nucleus Molecular Function:transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding Biological Process: transport; metabolic process; transmembrane transport
NCBI Summary:	This nuclear gene encodes a multi-pass membrane protein that is targeted to the mitochondrial inner membrane. The encoded protein is an ATP-dependent transporter that may mediate the passage of organic and inorganic molecules out of the mitochondria. Loss of function of the related gene in mouse results in a disruption of iron homeostasis between the mitochondria and cytosol. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Code:	Q9NUT2
NCBI GenInfo Identifier:	143811358
NCBI Gene ID:	11194
NCBI Accession:	Q9NUT2.3
UniProt Related Accession:	Q9NUT2
Molecular Weight:	80kDa
NCBI Full Name:	ATP-binding cassette sub-family B member 8, mitochondrial
NCBI Synonym Full Names:	ATP binding cassette subfamily B member 8
NCBI Official Symbol:	ABCB8
NCBI Official Synonym Symbols:	MABC1; M-ABC1; EST328128
NCBI Protein Information:	ATP-binding cassette sub-family B member 8, mitochondrial
UniProt Protein Name:	ATP-binding cassette sub-family B member 8, mitochondrial
UniProt Synonym Protein Names:	Mitochondrial ATP-binding cassette 1; M-ABC1
Protein Family:	ATP-binding cassette sub-family
UniProt Gene Name:	ABCB8
UniProt Entry Name:	ABCB8_HUMAN

	Western blot analysis of extracts of various cell lines, using ABCB8 antibody (CAB2653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human liver damage using ABCB8 antibody (CAB2653) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using ABCB8 antibody (CAB2653) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using ABCB8 antibody (CAB2653) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using ABCB8 Polyclonal Antibody (CAB2653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.