Cell Biology Antibodies 12

Anti-ABCB4 Antibody (CAB9835)

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SKU:
CAB9835
Product Type:
Antibody
Applications:
WB
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ABCB4 Antibody
Antibody SKU:CAB9835
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human ABCB4 (NP_061337.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human ABCB4 (NP_061337.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDLE AAKN GTAW RPTS AEGD FELG ISSK QKRK KTKT VKMI GVLT LFRY SDWQ DKLF MSLG TIMA IAHG SGLP LMMI VFGE MTDK FVDT AGNF SFPV NFSL SLLN PGKI LE
Gene ID:5244
Uniprot:P21439
Cellular Location:Apical cell membrane, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane raft, Multi-pass membrane protein, clathrin-coated vesicle
Calculated MW:135kDa/140kDa/141kDa
Observed MW:144kDa
Synonyms:ABCB4, ABC21, GBD1, ICP3, MDR2, MDR2/3, MDR3, PFIC-3, PGY3
Background:The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.
UniProt Protein Function:ABCB4: a transporter that mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human ABCB4 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. Interacts with HAX1. Defects are the cause of gallstones, and progressive familial intrahepatic cholestasis 3, an autosomal recessive liver disorder that progresses to cirrhosis and liver failure before adulthood. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Transporter, ABC family; Hydrolase; EC 3.6.3.44; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: intercellular canaliculus; membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function:ATPase activity, coupled to transmembrane movement of substances; xenobiotic-transporting ATPase activity; ATP binding

Biological Process: response to drug; transport; multidrug transport; xenobiotic transport; lipid metabolic process; cellular lipid metabolic process; transmembrane transport

Disease: Gallbladder Disease 1; Cholestasis, Progressive Familial Intrahepatic, 3; Cholestasis, Intrahepatic, Of Pregnancy 3

NCBI Summary:The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008]
UniProt Code:P21439
NCBI GenInfo Identifier:126302568
NCBI Gene ID:5244
NCBI Accession:P21439.2
UniProt Secondary Accession:P21439,Q14813, A0A2V7, A4D1D3, A4D1D4, A4D1D5, D6W5P3 D6W5P4,
UniProt Related Accession:P21439
Molecular Weight:
NCBI Full Name:Multidrug resistance protein 3
NCBI Synonym Full Names:ATP-binding cassette, sub-family B (MDR/TAP), member 4
NCBI Official Symbol:ABCB4  
NCBI Official Synonym Symbols:GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3  
NCBI Protein Information:multidrug resistance protein 3; P-glycoprotein 3; multiple drug resistance 3; ATP-binding cassette sub-family B member 4; P glycoprotein 3/multiple drug resistance 3; P-glycoprotein-3/multiple drug resistance-3
UniProt Protein Name:Multidrug resistance protein 3
UniProt Synonym Protein Names:ATP-binding cassette sub-family B member 4; P-glycoprotein 3
Protein Family:ABC transporter B family
UniProt Gene Name:ABCB4  
UniProt Entry Name:MDR3_HUMAN
Anti-ABCB4 Polyclonal Antibody (CAB9835)Western blot analysis of extracts of mouse brain, using ABCB4 antibody (CAB9835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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