Antibody Name:	Anti-ABCA3 Antibody
Antibody SKU:	CAB6862
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 43-203 of human ABCA3 (NP_001080.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 43-203 of human ABCA3 (NP_001080.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RLKI QSEN VPNA TIYP GQSI QELP LFFT FPPP GDTW ELAY IPSH SDAA KTVT ETVR RALV INMR VRGF PSEK DFED YIRY DNCS SSVL AAVV FEHP FNHS KEPL PLAV KYHL RFSY TRRN YMWT QTGS FFLK ETEG WHTT SLFP LFPN PGPR EPTS PDGG E
Gene ID:	21
Uniprot:	Q99758
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	24kDa/191kDa
Observed MW:	191kDa

Synonyms:

ABCA3, ABC-C, ABC3, EST111653, LBM180, SMDP3

Background:

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. The full transporter encoded by this gene may be involved in development of resistance to xenobiotics and engulfment during programmed cell death.

UniProt Protein Function:	ABCA3: Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol. Defects in ABCA3 are the cause of pulmonary surfactant metabolism dysfunction type 3 (SMDP3); also called pulmonary alveolar proteinosis due to ABCA3 deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Belongs to the ABC transporter superfamily. ABCA family.
UniProt Protein Details:	Protein type:Transporter, ABC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: extracellular space; membrane; plasma membrane; integral to membrane Molecular Function:ATPase activity, coupled to transmembrane movement of substances; transporter activity; ATP binding Biological Process: response to drug; metabolic process; transport; transmembrane transport Disease: Surfactant Metabolism Dysfunction, Pulmonary, 3
NCBI Summary:	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. The full transporter encoded by this gene may be involved in development of resistance to xenobiotics and engulfment during programmed cell death. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99758
NCBI GenInfo Identifier:	85700402
NCBI Gene ID:	21
NCBI Accession:	Q99758.2
UniProt Secondary Accession:	Q99758,Q54A95, Q6P5P9, Q92473, B2RU09,
UniProt Related Accession:	Q99758
Molecular Weight:	1704
NCBI Full Name:	ATP-binding cassette sub-family A member 3
NCBI Synonym Full Names:	ATP-binding cassette, sub-family A (ABC1), member 3
NCBI Official Symbol:	ABCA3
NCBI Official Synonym Symbols:	ABC3; ABC-C; SMDP3; LBM180; EST111653
NCBI Protein Information:	ATP-binding cassette sub-family A member 3; ABC transporter 3; ABC-C transporter; ATP-binding cassette transporter 3
UniProt Protein Name:	ATP-binding cassette sub-family A member 3
UniProt Synonym Protein Names:	ABC-C transporter; ATP-binding cassette transporter 3; ATP-binding cassette 3
Protein Family:	ABC transporter A family
UniProt Gene Name:	ABCA3
UniProt Entry Name:	ABCA3_HUMAN

	Western blot analysis of extracts of rat kidney, using ABCA3 antibody (CAB6862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using ABCA3 antibody (CAB6862). Blue: DAPI for nuclear staining.