Antibody Name:	Anti-ABCA2 Antibody
Antibody SKU:	CAB16735
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2307-2466 of human ABCA2 (NP_997698.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2307-2466 of human ABCA2 (NP_997698.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FGDG YMIT VRTK SSQS VKDV VRFF NRNF PEAM LKER HHTK VQYQ LKSE HISL AQVF SKME QVSG VLGI EDYS VSQT TLDN VFVN FAKK QSDN LEQQ ETEP PSAL QSPL GCLL SLLR PRSA PTEL RALV ADEP EDLD TEDE GLIS FEEE RAQL SFNT DTLC
Gene ID:	20
Uniprot:	Q9BZC7
Cellular Location:	Endosome membrane, Lysosome membrane, Multi-pass membrane protein
Calculated MW:	230kDa/269kDa
Observed MW:	270KDa

Synonyms:

ABCA2, ABC2

Background:

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is highly expressed in brain tissue and may play a role in macrophage lipid metabolism and neural development. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ABCA2: Probable transporter, its natural substrate has not been found yet. May have a role in macrophage lipid metabolism and neural development. Belongs to the ABC transporter superfamily. ABCA family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family; Transporter Chromosomal Location of Human Ortholog: 9q34 Cellular Component: cytoplasmic membrane-bound vesicle; endosome; integral to membrane; lysosome; membrane; microtubule organizing center Molecular Function:ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances Biological Process: cholesterol efflux; cholesterol homeostasis; phospholipid efflux; regulation of intracellular cholesterol transport; regulation of transcription from RNA polymerase II promoter; response to drug; response to steroid hormone stimulus
NCBI Summary:	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is highly expressed in brain tissue and may play a role in macrophage lipid metabolism and neural development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BZC7
NCBI GenInfo Identifier:	206729923
NCBI Gene ID:	20
NCBI Accession:	Q9BZC7.3
UniProt Secondary Accession:	Q9BZC7,Q5SPY5, Q5W9G5, Q76MW7, Q9HC28, A6NED5,
UniProt Related Accession:	Q9BZC7
Molecular Weight:	269,988 Da
NCBI Full Name:	ATP-binding cassette sub-family A member 2
NCBI Synonym Full Names:	ATP binding cassette subfamily A member 2
NCBI Official Symbol:	ABCA2
NCBI Official Synonym Symbols:	ABC2
NCBI Protein Information:	ATP-binding cassette sub-family A member 2
UniProt Protein Name:	ATP-binding cassette sub-family A member 2
UniProt Synonym Protein Names:	ATP-binding cassette transporter 2; ATP-binding cassette 2
Protein Family:	ABC transporter A family
UniProt Gene Name:	ABCA2
UniProt Entry Name:	ABCA2_HUMAN

	Western blot analysis of extracts of various cell lines, using ABCA2 Polyclonal Antibody (CAB16735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunofluorescence analysis of U-251 MG cells using ABCA2 Rabbit pAb (CAB16735) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse brain using ABCA2 Rabbit pAb (CAB16735) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.