Antibody Name:	Anti-AARS Antibody
Antibody SKU:	CAB15017
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AARS (NP_001596.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, Mouse brain, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AARS (NP_001596.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDST LTAS EIRQ RFID FFKR NEHT YVHS SATI PLDD PTLL FANA GMNQ FKPI FLNT IDPS HPMA KLSR AANT QKCI RAGG KHND LDDV GKDV YHHT FFEM
Gene ID:	16
Uniprot:	P49588
Cellular Location:	Cytoplasm
Calculated MW:	106kDa/109kDa
Observed MW:	107kDa

Synonyms:

AARS, CMT2N, EIEE29

Background:

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

UniProt Protein Function:	AARS: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. Defects in AARS are the cause of Charcot-Marie-Tooth disease type 2N (CMT2N). It is an axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies(designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Belongs to the class-II aminoacyl-tRNA synthetase family.
UniProt Protein Details:	Protein type:Aminoacyl-tRNA synthetase; EC 6.1.1.7; Ligase; RNA-binding; Translation; Translation regulation Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: cytoplasm; cytosol; membrane; mitochondrion Molecular Function:alanine-tRNA ligase activity; amino acid binding; tRNA binding Biological Process: alanyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; tRNA modification; tRNA processing Disease: Charcot-marie-tooth Disease, Axonal, Type 2n; Epileptic Encephalopathy, Early Infantile, 29
NCBI Summary:	The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008]
UniProt Code:	P49588
NCBI GenInfo Identifier:	115502460
NCBI Gene ID:	16
NCBI Accession:	P49588.2
UniProt Secondary Accession:	P49588,Q53GV7, Q96FA0, A6NF14, B4DR45,
UniProt Related Accession:	P49588
Molecular Weight:	107kDa
NCBI Full Name:	Alanine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:	alanyl-tRNA synthetase
NCBI Official Symbol:	AARS
NCBI Official Synonym Symbols:	CMT2N; EIEE29
NCBI Protein Information:	alanine--tRNA ligase, cytoplasmic
UniProt Protein Name:	Alanine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:	Alanyl-tRNA synthetase
UniProt Gene Name:	AARS

	Western blot analysis of extracts of various cell lines, using AARS Rabbit pAb (CAB15017) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat spleen using AARS antibody (CAB15017) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using AARS antibody (CAB15017) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using AARS antibody (CAB15017) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of 293T cells using AARS antibody (CAB15017) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.