Cell Biology Antibodies 2
Anti-AANAT Antibody (CAB11850)
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- SKU:
- CAB11850
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-AANAT Antibody |
| Antibody SKU: | CAB11850 |
| Antibody Size: | 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human AANAT |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human |
| Positive Samples: | 293T |
| Immunogen: | A synthetic peptide of human AANAT |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 15 |
| Uniprot: | Q16613 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 23kDa/28kDa |
| Observed MW: | 35kDa |
| Synonyms: | AANAT, DSPS, SNAT |
| Background: | The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | AA-NAT var1: Catalyzes the N-acetylation of serotonin into N- acetylserotonin. Controls the night/day rhythm of melatonin production in the pineal gland. Has a relative affinity for hydroxylated versus non-hydroxylated arylalkylamines. Belongs to the acetyltransferase family. AANAT subfamily. Monomer. Interacts with YWHAZ; the interaction requires phosphorylation on Thr-31, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-205 site, the other YWHAZ monomer with similar effect. |
| UniProt Protein Details: | Protein type:Acetyltransferase; EC 2.3.1.87; Amino Acid Metabolism - tryptophan Chromosomal Location of Human Ortholog: 17q25.1 Cellular Component: perinuclear region of cytoplasm; cytosol Molecular Function:arylamine N-acetyltransferase activity; aralkylamine N-acetyltransferase activity Biological Process: circadian rhythm; N-terminal protein amino acid acetylation; response to light stimulus; melatonin biosynthetic process; response to copper ion; response to zinc ion; indolalkylamine biosynthetic process; response to cytokine stimulus; response to calcium ion; response to corticosterone stimulus; response to insulin stimulus Disease: Delayed Sleep Phase Syndrome, Susceptibility To |
| NCBI Summary: | The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q16613 |
| NCBI GenInfo Identifier: | 11387096 |
| NCBI Gene ID: | 15 |
| NCBI Accession: | Q16613.1 |
| UniProt Secondary Accession: | Q16613,Q562F4, A0AVF2, J3KMZ5, |
| UniProt Related Accession: | Q16613 |
| Molecular Weight: | 28,432 Da |
| NCBI Full Name: | Serotonin N-acetyltransferase |
| NCBI Synonym Full Names: | aralkylamine N-acetyltransferase |
| NCBI Official Symbol: | AANAT |
| NCBI Official Synonym Symbols: | DSPS; SNAT |
| NCBI Protein Information: | serotonin N-acetyltransferase; serotonin acetylase; arylalkylamine N-acetyltransferase |
| UniProt Protein Name: | Serotonin N-acetyltransferase |
| UniProt Synonym Protein Names: | Aralkylamine N-acetyltransferase; AA-NAT |
| Protein Family: | Serotonin N-acetyltransferase |
| UniProt Gene Name: | AANAT |
| UniProt Entry Name: | SNAT_HUMAN |
 | Western blot analysis of extracts of 293T cells, using AANAT antibody (CAB11850) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
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