Signal Transduction Antibodies 1

Anti-AADAT Antibody (CAB13090)

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SKU:
CAB13090
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-AADAT Antibody
Antibody SKU:CAB13090
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 34-429 of human AADAT (NP_001273611.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 34-429 of human AADAT (NP_001273611.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PKSM ISLA GGLP NPNM FPFK TAVI TVEN GKTI QFGE EMMK RALQ YSPS AGIP ELLS WLKQ LQIK LHNP PTIH YPPS QGQM DLCV TSGS QQGL CKVF EMII NPGD NVLL DEPA YSGT LQSL HPLG CNII NVAS DESG IVPD SLRD ILSR WKPE DAKN PQKN TPKF LYTV PNGN NPTG NSLT SERK KEIY ELAR KYDF LIIE DDPY YFLQ FNKF RVPT FLSM DVDG RVIR ADSF SKII SSGL RIGF LTGP KPLI ERVI LHIQ VSTL HPST FNQL MISQ LLHE WGEE GFMA HVDR VIDF YSNQ KDAI LAAA DKWL TGLA EWHV PAAG MFLW IKVK GIND VKEL IEEK AVKM GVLM LPGN AFYV DSSA PSPY LRAS FSSA SPEQ MDVA FQVL AQLI KESL
Gene ID:51166
Uniprot:Q8N5Z0
Cellular Location:Mitochondrion
Calculated MW:47kDa
Observed MW:47kDa
Synonyms:AADAT, KAT2, KATII, KYAT2
Background:This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene.
UniProt Protein Function:AADAT: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino- group acceptors, with a preference for 2-oxoglutarate, 2- oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro). Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.6.1.7; Amino Acid Metabolism - tryptophan; EC 2.6.1.39; Amino Acid Metabolism - lysine degradation; Transferase; Amino Acid Metabolism - lysine biosynthesis; Mitochondrial

Chromosomal Location of Human Ortholog: 4q33

Cellular Component: mitochondrial matrix

Molecular Function:protein homodimerization activity; kynurenine-oxoglutarate transaminase activity; 2-aminoadipate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; tryptophan catabolic process to kynurenine; L-lysine catabolic process to acetyl-CoA via saccharopine; lysine catabolic process; biosynthetic process; tryptophan catabolic process; 2-oxoglutarate metabolic process

NCBI Summary:This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
UniProt Code:Q8N5Z0
NCBI GenInfo Identifier:33469970
NCBI Gene ID:51166
NCBI Accession:NP_872603.1
UniProt Secondary Accession:Q8N5Z0,Q9UL02, B3KP84,
UniProt Related Accession:Q8N5Z0
Molecular Weight:47,822 Da
NCBI Full Name:kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform b
NCBI Synonym Full Names:aminoadipate aminotransferase
NCBI Official Symbol:AADAT  
NCBI Official Synonym Symbols:KAT2; KATII; KYAT2  
NCBI Protein Information:kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
UniProt Protein Name:Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
UniProt Synonym Protein Names:2-aminoadipate aminotransferase; 2-aminoadipate transaminase (EC:2.6.1.39); Alpha-aminoadipate aminotransferase; AadAT; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase 2 (EC:2.6.1.7); Kynurenine--oxoglutarate transaminase II
Protein Family:Kynurenine/alpha-aminoadipate aminotransferase
UniProt Gene Name:AADAT  
UniProt Entry Name:AADAT_HUMAN
Anti-AADAT Antibody (CAB13090)Western blot analysis of extracts of various cell lines, using AADAT antibody (CAB13090) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-AADAT Antibody (CAB13090)Immunohistochemistry of paraffin-embedded human liver using AADAT Rabbit pAb (CAB13090) at dilution of 1:200 (40x lens).
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