Cell Health Assays
Annexin V-Biotin Apoptosis Detection Kit (CV0003)
- SKU:
- CV0003
- Product Type:
- Assay
- Detection Method:
- Fluorometric
- Sample Type:
- Adherent Cells, Suspension Cells
- Research Area:
- Apoptosis
- Research Area:
- Annexin
Description
Annexin V-Biotin Apoptosis Detection Kit
Annexin V Apoptosis Detection Kits are a convenient, easy-to-use and safe method for Apoptosis Detection. Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS).
Externalization of phosphatidylserine residues on the outer plasma membrane of apoptotic cells allows detection via Annexin V. Once the apoptotic cells are bound with labelled Annexin V, it can be visualized with fluorescent microscopy or cytometry.
Since loss of membrane integrity is a pathognomonic feature of necrotic cell death, necrotic cells will stain with specific membrane-impermeant nucleic acid dyes such as propidium iodide, the membrane integrity of apoptotic cells can be demonstrated by the exclusion of these dyes.
Features & Benefits
| Overview | Description |
| Size: | 100 Assays |
| Easy and one step protocol: | Get results in 9 minutes. |
| Detection Method: | Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy. |
| Reactivity: | Human, Mouse, Rat & other species |
| Proven performance: | Works with both adherent and suspension cells. |
| Safe: | Non-enzymatic assay that avoids fixation. |
Reagents Included:
| Buffer/Reagent | Volume |
| Labeled Annexin V | 500 µL |
| Binding Buffer (10x) | 50 mL |
| Propidium Iodide | 1 mL |
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