Antibody Name:	Angpt2 Antibody (PACO33756)
Antibody SKU:	PACO33756
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200
Species Reactivity:	Mouse
Immunogen:	Recombinant Mouse Angiopoietin-2 protein (19-483AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot All lanes: Angpt2 antibody at 2µg/ml + Mouse lung tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa .
	Immunohistochemistry of paraffin-embedded human placenta tissue using PACO33756 at dilution of 1:20.
	Immunohistochemistry of paraffin-embedded mouse kidney tissue using PACO33756 at dilution of 1:50.

Background:

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal

Synonyms:

Angiopoietin-2 (ANG-2), Angpt2, Agpt2

UniProt Protein Function:	ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Cellular Component: extracellular space; cell projection; plasma membrane; extracellular region; nucleus Molecular Function:protein binding; metal ion binding; receptor tyrosine kinase binding; vascular endothelial growth factor receptor binding Biological Process: Tie receptor signaling pathway; multicellular organismal development; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; negative regulation of positive chemotaxis; negative regulation of angiogenesis; positive regulation of angiogenesis; blood vessel morphogenesis; hemopoiesis; blood vessel remodeling; angiogenesis; cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; endoderm development
NCBI Summary:	This gene encodes an endothelial cell (EC)-derived regulator of angiogenesis and ligand for endothelial-specific receptor tyrosine kinase. The encoded protein acts as an anti-apoptotic factor for stressed ECs and a proapoptotic factor for resting ECs. [provided by RefSeq, Jan 2013]
UniProt Code:	O35608
NCBI GenInfo Identifier:	31982508
NCBI Gene ID:	11601
NCBI Accession:	NP_031452.2
UniProt Secondary Accession:	O35608,Q3U1A1, Q9D2D2,
UniProt Related Accession:	O35608
Molecular Weight:	56,576 Da
NCBI Full Name:	angiopoietin-2
NCBI Synonym Full Names:	angiopoietin 2
NCBI Official Symbol:	Angpt2
NCBI Official Synonym Symbols:	Ang2; Agpt2; Ang-2
NCBI Protein Information:	angiopoietin-2
UniProt Protein Name:	Angiopoietin-2
UniProt Gene Name:	Angpt2
UniProt Entry Name:	ANGP2_MOUSE