Cell Biology Assays

Ammonia Assay Kit (Fluorometric) (BA0054)

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SKU:
BA0054
Product Type:
Assay
Detection Method:
Fluorometric
Instrument:
Microplate Reader
Sample Type:
Biological, Environmental Samples
Research Area:
Urea
Research Area:
Food Safety & Analysis
Research Area:
Plant & Environmental Stress
Research Area:
Clinical Chemistry

Description

ELISA Kit Technical ManualMSDS

Ammonia Assay Kit - Information

Assay Genie's ammonia assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (Ex/Em = 360/450nm) is proportional to the ammonia concentration in the sample.

Applications

For quantitative determination of ammonia/ammonium concentration by fluorimetry at 360nm/450nm

Ammonia Assay Kit - Key Features

  • Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Ammonia Assay Kit - Data Sheet

Kit IncludesAssay Buffer: 20 mL Standard: 400 mL NH4Cl Reagent A: 1 mL Reagent B: 1 mL
Kit RequiresPipetting devices, centrifuge tubes, black flat bottom 96-well plates and plate reader.
Method of DetectionFL360/450nm
Detection Limit12
SamplesBiological (eg urine) and enviromental samples
SpeciesAll
Protocol Length15 min
Size200 tests
StorageStore kit at -20°C.
Shelf Life6 months

More Details

AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3 are very desirable.
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