Metabolism Assays
Alpha-Mannosidase Activity Assay Kit (BA0014)
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- SKU:
- BA0014
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Biological Samples Plasma, Serum, Tissue, Culture Media
- Research Area:
- Plant & Environmental Stress
- Research Area:
- Enzyme Activity
Description
alpha-Mannosidase Activity Assay Kit - Information
Assay Genie's non-radioactive, colorimetric alpha-Mannosidase activity assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.Applications
For quantitative determination of alpha-mannosidase enzyme activity using a p-nitrophenol based substrate.alpha-Mannosidase Activity Assay Kit - Key Features
- Fast and sensitive. Linear detection range (10 sample): 1 to 250 U/L for a 10 minute reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day.
alpha-Mannosidase Activity Assay Kit - Data Sheet | |
| Kit Includes | Substrate Buffer: 10 mL Stop Reagent: 12 mL Standard: 1 mL 12.5 mM Nitrophenol |
| Kit Requires | Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well plates, centrifuge tubes and plate reader. |
| Method of Detection | OD405nm |
| Detection Limit | 1 U/L |
| Samples | Biological samples e.g. plasma, serum, tissue and culture media. |
| Species | All |
| Protocol Length | 10 min |
| Size | 100 tests |
| Storage | Store all components at 4°C upon receiving. |
| Shelf Life | 1 U/L |
More Details
alpha-MANNOSIDASE (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. alpha-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes alpha-mannosidosis and leads to deterioration of the central nervous system in children.Related Products
Alpha-Amylase and Beta-amylase Activity Assay Kit (MAES0019)
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