alpha-L- Fucosidase Assay Kit (AFU) (BA0023)

alpha-L- Fucosidase Assay Kit (AFU) - Information

Assay Genie' non-radioactive, colorimetric AFU assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

Applications

For quantitative determination of alpha-L-Fucosidase activity in biological samples (e.g. plasma, serum, tissue, cell lysate, etc.)

alpha-L- Fucosidase Assay Kit (AFU) - Key Features

• High sensitivity and wide linear range. Linear detection range (10 sample): 1 to 100 U/L.
• Homogeneous and simple procedure. Simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes.
• Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Can be readily automated to measure thousands of samples per day.

alpha-L- Fucosidase Assay Kit (AFU) - Data Sheet
Kit Includes	Substrate Buffer: 10 mL Stop Reagent: 12 mL Standard: 1 mL 12.5 mM Nitrophenol
Kit Requires	Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well plates, centrifuge tubes and plate reader
Method of Detection	OD405nm
Detection Limit	1 U/L
Samples	Plasma, serum, tissue, cell lysate, etc.
Species	All
Protocol Length	20 min
Size	100 tests
Shelf Life	6 Months
Storage	Store all components at 4°C upon receiving

More Details

alpha-L-FUCOSIDASE (AFU) is an enzyme coded by the FUCA1 gene in humans and catalyzes the breakdown of L-Fucose. A genetic deficiency in this enzyme results in a neurovisceral storage disease, fucosidosis, which is characterized by the accumulation of fucose. Low serum activity of fucosidase has also been linked to ovarian caricinoma. Elevated fucosidase serum activity has been observed in patients with diabetes, hyperthyroidism, cirrhosis, and hepatitis. Increased activity has been associated with lung, breast, stomach, ovary, uterus, and liver carcinomas.

Aliases for FUCA1 Gene

• Fucosidase, Alpha-L- 1, Tissue
• Alpha-L-Fucoside Fucohydrolase 1
• Alpha-L-Fucosidase 1
• Alpha-L-Fucosidase I
• EC 3.2.1.51
• Tissue Alpha-L-Fucosidase
• EC 3.2.1
• FUCA

Entrez Gene Summary for FUCA1 Gene

The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]

UniProtKB/Swiss-Prot for FUCA1 Gene

• FUCO_HUMAN,P04066
• Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.

Protein details for FUCA1 Gene (UniProtKB/Swiss-Prot)

Protein Symbol:P04066-FUCO_HUMANRecommended name:Tissue alpha-L-fucosidaseProtein Accession:P04066Secondary Accessions:B2RBG3,Q14334, Q14335, Q3LID0, Q8NAC2

Protein attributes for FUCA1 Gene

Size:466 amino acidsMolecular mass:53689 DaQuaternary structure:Homotetramer.SequenceCaution:Sequence=AAA35519.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAA52481.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAH17338.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAG37210.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA25646.1; Type=Miscellaneous discrepancy; Note=Translation of X01390 sequence produces a larger peptide than that shown in CAA25646.1.; Evidence={ECO:0000305};

Post-translational modifications for FUCA1 Gene

• Glycosylation at Asn 241, Asn 268, and Asn 382

• NX_P04066

• Modification sites at PhosphoSitePlus

• P04066