Description
| Product Name: | Alpha-Ketoglutarate Fluorometric Assay Kit |
| Product Code: | MAES0258 |
| Sample Type: | Serum, Plasma, Urine, Animal Tissue And Cell Sample |
| Assay Time: | 35 min |
| Assay Type: | Quantitative |
| Synonyms: | α-KG |
| Detection Instrument: | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Detection Method: | Fluorescence method |
| Sensitivity: | 0.60 μmol/L |
| Detection Range: | 0.60-100 μmol/L |
| Inter-assay CV: | 0.04 |
| Intra-assay CV: | 0.02 |
α-Ketoglutaric acid and alanine can be combined with fluorescent probe products under the action of a series of enzymes. The content of α-ketoglutaric acid in samples can be determined by measuring the fluorescence value.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
| Other Instruments Required: | Incubator, Centrifuge, Fluorescence microplate reader (Ex/Em=535 nm/587 nm), 50 KD ultrafiltration tube |