Alkaline Phosphatase Activity Assay Kit (MAES0023)
- SKU:
- MAES0023
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Animal Tissue, Cells
- Sensitivity:
- 0.27 U/L
- Range:
- 0.27-50.8 U/L
- Assay Time:
- 30 min
- Shipping:
- Gel Pack
- Research Area:
- Enzyme Activity
Description
system_update_altDatasheet
| Product Name: | Alkaline Phosphatase Activity Assay Kit |
| Product Code: | MAES0023 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue |
| Assay Time: | 30 min |
| Instrument: | Microplate reader |
| Sensitivity: | 0.27 U/L |
| Detection Range: | 0.27-50.8 U/L |
| Inter CV: | 4.6 |
| Inter CV: | 1.2 |
Alkaline phosphatase (ALP) is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase and each catalytic site contains three metal ions. There are four isozymes in humans: tissue nonspecific ALP, intestinal ALP, placental ALP and genital cell ALP.
Under alkaline conditions, alkaline phosphatase catalyzes the hydrolysis of p- nitrobenzene phosphate disodium to produce p-nitrophenol and phosphoric acid. Under strong alkaline conditions, p-nitrophenol is bright yellow and has a maximum absorption peak at 405 nm. Therefore, the activity of ALP can be calculated by measuring the OD value at 405 nm.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Enzymes, Liver Biomarkers |
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