Aldehyde Dehydrogenase (ALDH) Fluorometric Activity Assay Kit

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SKU:
MAES0221
Product Type:
Assay
Instrument:
Fluorescence Microplate Reader
Detection Method:
Fluorometric
Sample Type:
Serum, Plasma, Animal Tissue, Cell
Sensitivity:
0.06 μmol/L
Range:
0.06-3 μmol/L
Assay Time:
20 min

Description

Product Name:Aldehyde Dehydrogenase (ALDH) Fluorometric Activity Assay Kit
Product Code:MAES0221
Sample Type:Serum (Plasma), Animal Tissue, Cell
Assay Time:20 min
Assay Type:Enzyme Activity
Synonyms:ALDH
Detection Instrument:Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Detection Method:Fluorescence method
Sensitivity:0.06 μmol/L
Detection Range:0.06–3 μmol/L
Inter-assay CV:0.084
Intra-assay CV:0.04

The detection principle of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD+ into NADH, NADH reacts with the fluorescent probe to form fluorescent substance under the action of enzymes. The activity of ALDH can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.

Storage:-20°C
Valid Period:12 months
Other Instruments Required:Micropipettor, Centrifuge
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