Description
Product Name: | Aldehyde Dehydrogenase (ALDH) Activity Assay Kit |
Product Code: | MAES0228 |
Sample Type: | Serum (Plasma), Animal Tissue |
Assay Time: | 80 min |
Assay Type: | Enzyme Activity |
Synonyms: | ALDH |
Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
Detection Method: | Colorimetric method |
Sensitivity: | 0.01 U/L |
Detection Range: | 0.01–10.0 U/L |
Inter-assay CV: | 0.073 |
Intra-assay CV: | 0.03 |
The detection principle of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD+ into NADH, which under the action of electron coupling agent, transfer electrons to WST-8 to produce the yellow product. The activity of ADH can be calculated by measuring the change of absorbance value at 450 nm.
Storage: | -20°C |
Valid Period: | 12 months |
Other Instruments Required: | Incubator, Centrifuge |