Description
Product Name: | Alcohol Dehydrogenase (ADH) Activity Assay Kit |
Product Code: | MAES0216 |
Sample Type: | Serum, Plasma, Animal Tissue |
Assay Time: | 30 min |
Assay Type: | Enzyme Activity |
Synonyms: | ADH |
Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
Detection Method: | Colorimetric method |
Sensitivity: | 0.29 U/L |
Detection Range: | 0.29-248 U/L |
Inter-assay CV: | 0.045 |
Intra-assay CV: | 0.042 |
ADH catalyzes the oxidative dehydrogenation of ethanol. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of ADH can be calculated by measuring the change of absorbance value at 450 nm.
Storage: | -20°C |
Valid Period: | 12 months |
Other Reagents Required: | Normal saline (0.9% NaCl) |
Other Instruments Required: | Incubator, centrifuge, Microplate reader |