Alanine Transaminase Assay Kit (BA0074)

Alanine Transaminase Assay Kit - Information

Assay Genie's alanine transaminase activity assay (ALT) is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340 nm is proportional to ALT activity.

Applications

For quantitative determination of alanine transaminase activity.

Alanine Transaminase Assay Kit - Key Features

• Sensitive. Linear detection range: 2-100 U/L.
• Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min.

Alanine Transaminase Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 24 mL LDH: 120 uL Cosubstrate: 600 uL NADH Reagent: Dried
Kit Requires	Pipeting devices and accessories. Clear bottom 96-well plates and plate reader or spectrophotometer and cuvettes for measuring OD340nm.
Method of Detection	OD340nm
Detection Limit	2 U/L
Samples	Serum, plasma etc
Species	All
Protocol Length	10 min
Size	100 tests
Storage	Store all components at -20°C
Shelf Life	6 months

More Details

Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Simple, direct and automation-ready procedures for measuring ALT activity find wide applications in research and drug discovery.