](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/12571/16623/afle-purified-mouse-cd279pd-1-monoclonal-antibody-29f.1a12agel1624__27381__64932.1706270068.jpg?c=1?imbypass=on "AF/LE Purified Mouse CD279/PD-1 Monoclonal Antibody [29F.1A12](AGEL1624)")
Description
| Antibody Name: | AF/LE Purified Mouse CD279/PD-1 Monoclonal Antibody |
| Antibody Code: | AGEL1624 |
| Size: | 500µg |
| Clone: | 29F.1A12 |
| Synonyms: | PD-1, Programmed Death-1 |
| Host Species: | Rat |
| Species Reactivity: | Mouse |
| Applications: | Block, Neut, FC, WB, IHC, IF |
| Concentration: | 1mg/mL |
| Isotype: | IgG2a,k |
| Conjugation: | Azide Free/Low endotoxin |
| Applications: | Block, Neut, FC, WB, IHC, IF |
| Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Storage Buffer: | 0.2 µm filtered in PBS, pH7.2. Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg (< 0.002EU/µg) as Determined by LAL gel clotting assay. Purity is > 95% as determined by SDS-PAGE |
| Storage: | Store at 2-8°C. Do not freeze. |
| Gene ID: | 18566 |
| Uniprot: | Q02242 |
| Background: | CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts. |
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