AF/LE Purified Anti-Mouse CD22 Antibody [Cy34.1]

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SKU:
AGEL2157
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FCM
Reactivity:
Mouse
Host Species:
Mouse
Isotype:
Mouse IgG1, κ
Clone:
Cy34.1
Conjugation:
None (Low endotoxin)

Description

Product Name:AF/LE Purified Anti-Mouse CD22 Antibody [Cy34.1]
Product Code:AGEL2157
Clone No:Cy34.1
Synonyms:B-cell receptor CD22,Cd22,B-lymphocyte cell adhesion molecule,BL-CAM,Sialic acid-binding Ig-like lectin 2,Siglec-2,T-cell surface antigen Leu-14,CD22,Lyb-8, Siglec2
Reactivity:Mouse
Host Species:Mouse
Applications:FCM
Isotype:Mouse IgG1, κ
Isotype Control:Low Endotoxin Purified Mouse IgG1, κ Isotype Control [MOPC-21] (AGEL2559)
Conjugation:None (Purified antibody-Azide Free/Low endotoxin)
Form:Liquid
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Swissprot:P35329
Stability & Storage:Keep as concentrated solution. Store at 2-8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Shipping:Biological ice pack at 4°C

The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.

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