AF/LE Purified Anti-Human/Mouse/Rat CD47 Antibody [MIAP410]

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SKU:
AGEL2153
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FCM
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Mouse
Isotype:
Mouse IgG1, κ
Clone:
MIAP410
Conjugation:
None (Low endotoxin)

Description

Product Name:AF/LE Purified Anti-Human/Mouse/Rat CD47 Antibody [MIAP410]
Product Code:AGEL2153
Clone No:MIAP410
Synonyms:Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP
Reactivity:Human,Mouse,Rat
Host Species:Mouse
Applications:FCM
Isotype:Mouse IgG1, κ
Isotype Control:Low Endotoxin Purified Mouse IgG1, κ Isotype Control [MOPC-21] (AGEL2559)
Conjugation:None (Purified antibody-Azide Free/Low endotoxin)
Form:Liquid
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Swissprot:Q08722,Q61735,P97829
Stability & Storage:Keep as concentrated solution. Store at 2-8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Shipping:Biological ice pack at 4°C

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

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