ADO Antibody (PACO17495)
- SKU:
- PACO17495
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ADO Antibody (PACO17495) |
| Antibody SKU: | PACO17495 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:1000-1:10000, WB:1:1000-1:5000, IHC:1:25-1:100 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Synthetic peptide of human ADO |
| Form: | Liquid |
| Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Purification Method: | Antigen affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane: Mouse testis tissue, Primary antibody: PACO17495(ADO Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 minutes. |
 | The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO17495(ADO Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200). |
| Background: | Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity. |
| Synonyms: | 2-aminoethanethiol (cysteamine) dioxygenase |
| UniProt Protein Function: | ADO: |
| UniProt Protein Details: | Protein type:EC 1.13.11.19; Oxidoreductase; Other Amino Acids Metabolism - taurine and hypotaurine Chromosomal Location of Human Ortholog: 10q21.3 Cellular Component: mitochondrion Molecular Function:metal ion binding; cysteamine dioxygenase activity |
| NCBI Summary: | Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine (Dominy et al., 2007 [PubMed 17581819]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q96SZ5 |
| NCBI GenInfo Identifier: | 88984104 |
| NCBI Gene ID: | 84890 |
| NCBI Accession: | Q96SZ5.2 |
| UniProt Related Accession: | Q96SZ5 |
| Molecular Weight: | 30kDa |
| NCBI Full Name: | 2-aminoethanethiol dioxygenase |
| NCBI Synonym Full Names: | 2-aminoethanethiol dioxygenase |
| NCBI Official Symbol: | ADO |
| NCBI Official Synonym Symbols: | C10orf22 |
| NCBI Protein Information: | 2-aminoethanethiol dioxygenase |
| UniProt Protein Name: | 2-aminoethanethiol dioxygenase |
| UniProt Synonym Protein Names: | Cysteamine dioxygenase |
| Protein Family: | AdoMet-dependent rRNA methyltransferase |
| UniProt Gene Name: | ADO |
| UniProt Entry Name: | AEDO_HUMAN |
| Antibodies |
| ADO Antibody (PACO07622) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
