Acid Phosphatase Activity Assay Kit (MAES0082)

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SKU:
MAES0082
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Hydrothorax, Urine, Cells, Cell Culture Supernatant, Animal Tissue
Sensitivity:
1.40 U/100 mL
Range:
1.40-40 U/100 mL
Assay Time:
45 min
Shipping:
Gel Pack
Research Area:
Diabetes & Obesity
Research Area:
Enzyme Activity

Description

system_update_altDatasheet
Product Name:Acid Phosphatase Activity Assay Kit
Product Code:MAES0082
Product Size:96 Assays
Sample Type:Serum, plasma, hydrothorax, urine, cells, cell culture supernatant, animal tissue
Assay Time:45 min
Instrument:Microplate reader
Sensitivity:1.40 U/100 mL
Detection Range:1.40-40 U/100 mL
Recovery Rate:104
Inter CV:9.4
Inter CV:2.1

Acid phosphatase (ACP) is a kind of enzyme which catalyzes the hydrolysis of phosphate monoester to phosphoric acid under acidic conditions. There are different acid phosphatase isozymes in different organs. These isozymes differ greatly in tissue and chromosome origin, molecular weight, amino acid homology, sequence length, and resistance to L-tartrate or fluoride.

Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.

Shelf Life:3 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Enzymes, Others
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