Acetylcholine Assay Kit (BA0069)

Acetylcholine Assay Kit - Information

Assay Genie's acetylcholine assay provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H₂O₂. The resulting H₂O₂ reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.

Applications

For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism.

Acetylcholine Assay Kit - Key Features

• Use 20 samples. Linear detection range: colorimetric assay 10 to 200 , fluorimetric assay 0.4 to 10 acetylcholine.

Acetylcholine Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 10 mL ACHE Enzyme: 120 mL Enzyme Mix: Dried Dye Reagent: 120 mL Standard: 400 mL 2 mM acetylcholine
Kit Requires	Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader.
Method of Detection	OD570nm, or FL530/585nm
Detection Limit	OD, FL: 10, 0.4
Samples	Serum, plasma, urine, saliva, milk, tissue, and cell culture etc
Species	All
Protocol Length	30 min
Size	100 tests
Storage	Store all components at -20°C.
Shelf Life	6 months

More Details

ACETYLCHOLINE is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood.