Description
Product Name: | Acetyl-Histone H3-K9 Monoclonal Antibody |
Product Code: | CAB21107 |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Applications: | Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
Host Species: | Rabbit |
Purification Method: | Affinity purification |
Isotype: | IgG |
Clone No: | ARC50576 |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Tested Applications: | WB, IHC-P, IF/ICC, IP, ChIP, ELISA, DB, CUT&Tag |
Key Applications: | Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
Recommended Dilution: | DB 1:500-1:1000, WB 1:500-1:1000, IHC-P 1:100-1:500, IF/ICC 1:50-1:200, IP 1:500-1:1000, ChIP 1:50-1:200, CUT&Tag 10, ⁵ cells /1 μg |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Positive Samples: | HeLa, NIH3T3, C6 |
Cellular Location: | Chromosome, Nucleus |
Customer Validation: | WB(Homo sapiens) |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Immunogen: | A synthetic acetylated peptide around K9 of human Histone H3 (NP_003520.1). |
Sequence: | MARTK QTARK STGGK APRKQ LATKA ARKSA PATGG VKKPH RYRPG TVALR EIRRY QKSTE LLIRK LPFQR LVREI AQDFK TDLRF QSSAV MALQE ACEAY |
Synonyms: | H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A |
Calculated MW: | 15kDa |
Observed MW: | 17kDa |