Description
Product Name: | Acetyl-Histone H3-K4 Monoclonal Antibody |
Product Code: | CAB24341 |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Applications: | Western blotting, Immunohistochemistry, Immunoprecipitation |
Host Species: | Rabbit |
Purification Method: | Affinity purification |
Isotype: | IgG |
Clone No: | ARC62519 |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Tested Applications: | WB, IHC-P, IP, ChIP, ELISA, DB |
Key Applications: | Western blotting, Immunohistochemistry, Immunoprecipitation |
Recommended Dilution: | DB 1:2000-1:5000, WB 1:500-1:1000, IHC-P 1:50-1:200, ChIP 1:50-1:200, IP 1:50-1:200 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Positive Samples: | HeLa TSA, NIH/3T3 TSA, C6 TSA |
Cellular Location: | Chromosome, Nucleus |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Immunogen: | A synthetic acetylated peptide around K4 of human Histone H3 (NP_003520.1). |
Sequence: | ARTK( AC)QT ARKST GGK-C |
Synonyms: | H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3 |
Calculated MW: | 15kDa |
Observed MW: | 17kDa |