Antibody Name:	AC1 Antibody (PACO34286)
Antibody SKU:	PACO34286
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA
Recommended Dilutions:
Species Reactivity:	African cassava mosaic virus
Immunogen:	Recombinant African cassava mosaic virus Replication-associated protein (1-358AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Background:

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).

Synonyms:

Replication-associated protein (Rep) (EC 2.7.7.-) (EC 3.1.21.-) (40.4 kDa protein) (Protein AC1) (Protein AL1)

UniProt Protein Function:	Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities ().
UniProt Protein Details:
NCBI Summary:
UniProt Code:	P14982
NCBI GenInfo Identifier:	137408
NCBI Gene ID:
NCBI Accession:	P14982.1
UniProt Secondary Accession:	P14982
UniProt Related Accession:	P14982
Molecular Weight:	42.3 kDa
NCBI Full Name:	Replication-associated protein
NCBI Synonym Full Names:
NCBI Official Symbol:
NCBI Official Synonym Symbols:
NCBI Protein Information:
UniProt Protein Name:	Replication-associated protein
UniProt Synonym Protein Names:	40.4 kDa protein; Protein AC1; Protein AL1
Protein Family:	Replication-associated protein
UniProt Gene Name:	AC1
UniProt Entry Name:	REP_CLVK