Name: ABLIM1 Antibody (PACO47274)
SKU: PACO47274
Availability: OutOfStock

Antibody Name:	ABLIM1 Antibody (PACO47274)
Antibody SKU:	PACO47274
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant Human Actin-binding LIM protein 1 protein (174-319AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, Mouse heart tissue. All lanes: ABLIM1 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 88, 82, 49, 47, 53, 85 kDa. Observed band size: 88 kDa..
	Immunofluorescent analysis of HepG2 cells using PACO47274 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Immunohistochemistry of paraffin-embedded human heart tissue using PACO47274 at dilution of 1:100.

Background:	May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.
Synonyms:	Actin-binding LIM protein 1 (abLIM-1) (Actin-binding LIM protein family member 1) (Actin-binding double zinc finger protein) (LIMAB1) (Limatin), ABLIM1, ABLIM KIAA0059 LIMAB1

UniProt Protein Function:	abLIM: a cytoskeletal LIM protein consisting of a C-terminal cytoskeletal domain fused to an N-terminal domain of four double zinc finger motifs. The C-terminal domain is 50% identical to dematin, an actin-bundling protein of the erythroid cytoskeleton. Undergoes extensive phosphorylation in light-adapted retinas in vivo and its developmental expression in the retina coincides with the elaboration of photoreceptor inner and outer segments. Three distinct splice variant isoforms have been described: ABLIM-l, ABLIM-m, ABLIM-s.Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Actin-bindingChromosomal Location of Human Ortholog: 10q25Cellular Component: lamellipodium; cytoplasm; stress fiber; actin cytoskeletonMolecular Function: protein binding; zinc ion binding; actin bindingBiological Process: lamellipodium biogenesis; axon guidance; organ morphogenesis; visual perception; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; cytoskeleton organization and biogenesis
UniProt Protein Details:
NCBI Summary:	This gene encodes a cytoskeletal LIM protein that binds to actin filaments via a domain that is homologous to erythrocyte dematin. LIM domains, found in over 60 proteins, play key roles in the regulation of developmental pathways. LIM domains also function as protein-binding interfaces, mediating specific protein-protein interactions. The protein encoded by this gene could mediate such interactions between actin filaments and cytoplasmic targets. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	O14639
NCBI GenInfo Identifier:	206729924
NCBI Gene ID:	3983
NCBI Accession:	O14639.3
UniProt Secondary Accession:	O14639,Q15039, Q5JVV1, Q5JVV2, Q5T6N3, Q5T6N5, Q68CQ9 Q9BUP1, A6NI16, A6NJ06
UniProt Related Accession:	O14639
Molecular Weight:	52,056 Da
NCBI Full Name:	Actin-binding LIM protein 1
NCBI Synonym Full Names:	actin binding LIM protein 1
NCBI Official Symbol:	ABLIM1
NCBI Official Synonym Symbols:	ABLIM; LIMAB1; LIMATIN; abLIM-1
NCBI Protein Information:	actin-binding LIM protein 1; actin-binding double-zinc-finger protein; actin-binding LIM protein family member 1
UniProt Protein Name:	Actin-binding LIM protein 1
UniProt Synonym Protein Names:	Actin-binding LIM protein family member 1; Actin-binding double zinc finger protein; LIMAB1; Limatin
Protein Family:	Actin-binding LIM protein
UniProt Gene Name:	ABLIM1
UniProt Entry Name:	ABLM1_HUMAN