AACS Antibody (PACO53954)
- SKU:
- PACO53954
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | AACS Antibody (PACO53954) |
| Antibody SKU: | PACO53954 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Acetoacetyl-CoA synthetase protein (435-598AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: HEK293 whole cell lysate. All lanes: AACS antibody at 2.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 76, 68, 31 kDa. Observed band size: 76 kDa. |
 | Immunoprecipitating AACS in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of PACO53954 in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO53954 (8µg) + HEK293 whole cell lysate (500µg). Lane 3: HEK293 whole cell lysate (10µg). |
 | Immunohistochemistry of paraffin-embedded human kidney tissue using PACO53954 at dilution of 1:100. |
| Background: | Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid, synthesis during adipose tissue development (By similarity). |
| Synonyms: | Acetoacetyl-CoA synthetase (EC 6.2.1.16) (Acyl-CoA synthetase family member 1) (Protein sur-5 homolog), AACS, ACSF1 |
| UniProt Protein Function: | AACS: Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. Belongs to the ATP-dependent AMP-binding enzyme family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Ligase; EC 6.2.1.16; Carbohydrate Metabolism - butanoateChromosomal Location of Human Ortholog: 12q24.31Cellular Component: cytosolMolecular Function: acetoacetate-CoA ligase activity; protein bindingBiological Process: ketone body biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q86V21 |
| NCBI GenInfo Identifier: | 74750446 |
| NCBI Gene ID: | 65985 |
| NCBI Accession: | Q86V21.1 |
| UniProt Secondary Accession: | Q86V21,Q49AB9, Q49AC3, Q658Q8, Q8IWD2, Q8NEW5, Q9BSJ9 Q9H829, Q9HA19 |
| UniProt Related Accession: | Q86V21 |
| Molecular Weight: | 30,061 Da |
| NCBI Full Name: | Acetoacetyl-CoA synthetase |
| NCBI Synonym Full Names: | acetoacetyl-CoA synthetase |
| NCBI Official Symbol: | AACS |
| NCBI Official Synonym Symbols: | ACSF1; SUR-5 |
| NCBI Protein Information: | acetoacetyl-CoA synthetase |
| UniProt Protein Name: | Acetoacetyl-CoA synthetase |
| UniProt Synonym Protein Names: | Acyl-CoA synthetase family member 1; Protein sur-5 homolog |
| Protein Family: | Acetoacetyl-CoA synthetase |
| UniProt Gene Name: | AACS |
| UniProt Entry Name: | AACS_HUMAN |
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